Human IFN-gamma Quantikine ELISA Kit
Human IFN-gamma Quantikine ELISA Kit
Human IFN-gamma Quantikine ELISA Kit Summary
Catalog # Availability Size / Price
DIF50 1 Kit / Please Inquire
SIF50 1 Pack (6 Plates) / Please Inquire
PDIF50 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant human IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
79.2
|
214
|
466
|
78.7
|
206
|
457
|
|
Standard Deviation
|
3.7
|
5.5
|
13
|
6.1
|
13.1
|
17
|
|
CV%
|
4.7
|
2.6
|
2.8
|
7.8
|
6.4
|
3.7
|
Recovery
The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
93-109
|
|
EDTA Plasma (n=5)
|
98
|
88-111
|
|
Serum (n=5)
|
102
|
91-118
|
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