Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
Catalog Size / Price
DLB50 1 Kit / Please Inquire
SLB50 1 Pack (6 Plates) / Please Inquire
PDLB50 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
4
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
18.9
|
30.2
|
122
|
181
|
29.8
|
118
|
193
|
|
Standard Deviation
|
1.6
|
1
|
5.4
|
5
|
2.5
|
5
|
8
|
|
CV%
|
8.5
|
3.3
|
4.4
|
2.8
|
8.4
|
4.2
|
4.1
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
4
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
12.6
|
30.4
|
69.2
|
192
|
30.8
|
120
|
194
|
|
Standard Deviation
|
0.3
|
1
|
1.6
|
6.5
|
2.2
|
4.1
|
7.9
|
|
CV%
|
2.4
|
3.3
|
2.3
|
3.4
|
7.1
|
3.4
|
4.1
|
Recovery
The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
97
|
80-111
|
|
Citrate Plasma (n=10)
|
93
|
83-110
|
|
EDTA Plasma (n=10)
|
86
|
81-100
|
|
Heparin Plasma (n=10)
|
82
|
76-100
|
|
Serum (n=10)
|
95
|
87-110
|
