Human CD14 Quantikine ELISA Kit
Human CD14 Quantikine ELISA Kit
Human CD14 Quantikine ELISA Kit Summary
Catalog # Size
DC140 1 Kit
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Assay Range
250.0 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sCD14
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1111
|
2158
|
4187
|
1159
|
2298
|
4346
|
|
Standard Deviation
|
71
|
104
|
216
|
86
|
153
|
209
|
|
CV%
|
6.4
|
4.8
|
5.2
|
7.4
|
6.7
|
4.8
|
Recovery
The recovery of sCD14 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=5)
|
91
|
85-105
|
|
Citrate Plasma (n=5)
|
102
|
88-110
|
|
EDTA Plasma (n=5)
|
98
|
90-107
|
|
Heparin Plasma (n=5)
|
96
|
91-104
|
|
Serum (n=5)
|
98
|
84-107
|
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of sCD14 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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