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          Human IL-2 Quantikine ELISA Kit


          

          
Human IL-2 Quantikine ELISA Kit
          Human IL-2 Quantikine ELISA Kit
          
            Human IL-2 Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	
          
		Human IL-2 Quantikine ELISA Kit Summary
	
          
	
          
		貨號(hào)：D2050
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					4.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					7 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human IL-2
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
			
          
			
          
				
					Mean
				
				
					67.5
				
				
					438
				
				
					851
				
				
					232
				
				
					620
				
				
					1148
				
			
          
			
          
				
					Standard Deviation
				
				
					2.9
				
				
					14.4
				
				
					26.9
				
				
					23.2
				
				
					34.4
				
				
					45.5
				
			
          
			
          
				
					CV%
				
				
					4.3
				
				
					3.3
				
				
					3.2
				
				
					10
				
				
					5.5
				
				
					4
				
			
          
		          
	
          

          
	
          
		Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
			
          
			
          
				
					Mean
				
				
					98
				
				
					619
				
				
					1183
				
				
					250
				
				
					755
				
				
					1470
				
			
          
			
          
				
					Standard Deviation
				
				
					4.2
				
				
					18
				
				
					24
				
				
					12.6
				
				
					27.8
				
				
					74.1
				
			
          
			
          
				
					CV%
				
				
					4.3
				
				
					2.9
				
				
					2
				
				
					5
				
				
					3.7
				
				
					5
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of human IL-2 spiked to three levels throughout the range of the assay was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=5)
				
				
					101
				
				
					96-107
				
			
          
			
          
				
					Citrate Plasma (n=5)
				
				
					105
				
				
					98-111
				
			
          
			
          
				
					EDTA Plasma (n=5)
				
				
					118
				
				
					104-139
				
			
          
			
          
				
					Heparin Plasma (n=5)
				
				
					109
				
				
					94-129
				
			
          
			
          
				
					Serum (n=5)
				
				
					99
				
				
					89-114
				
			
          
		          
	
          

          

          
	
          
		Linearity
	
          
	
          
		To assess the linearity of the assay, five samples were spiked with high concentrations of IL-2 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
	
          
	
          
		Human IL-2 ELISA Linearity
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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