Human Leptin Quantikine ELISA Kit
Human Leptin Quantikine ELISA Kit
Human Leptin Quantikine ELISA Kit Summary
貨號：DLP00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human Leptin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural human Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
64.5
|
146
|
621
|
65.7
|
146
|
581
|
|
Standard Deviation
|
2.14
|
4.32
|
20
|
3.56
|
6.17
|
20.6
|
|
CV%
|
3.3
|
3
|
3.2
|
5.4
|
4.2
|
3.5
|
Recovery
The recovery of Leptin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
98
|
94-102
|
|
Citrate Plasma (n=5)
|
95
|
87-105
|
|
EDTA Plasma (n=5)
|
99
|
85-112
|
|
Heparin Plasma (n=5)
|
90
|
81-100
|
|
Serum (n=5)
|
95
|
89-109
|
Linearity
To assess the linearity of the assay, cell culture media samples spiked with high concentrations of Leptin and natural serum/plasma samples were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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