Human HGF Quantikine ELISA Kit
Human HGF Quantikine ELISA Kit
Human HGF Quantikine ELISA Kit Summary
貨號(hào)：DHG00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.25 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)
Assay Range
125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human HGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
339
|
999
|
3759
|
363
|
967
|
3790
|
|
Standard Deviation
|
23.7
|
56.2
|
154
|
25.8
|
81.2
|
205
|
|
CV%
|
7
|
5.6
|
4.1
|
7.1
|
8.4
|
5.4
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
425
|
991
|
3869
|
409
|
1058
|
4058
|
|
Standard Deviation
|
30.2
|
43.2
|
234
|
29.4
|
75.1
|
264
|
|
CV%
|
7.1
|
4.4
|
6
|
7.2
|
7.1
|
6.5
|
Recovery
The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
92
|
86-101
|
|
Citrate Plasma (n=5)
|
105
|
99-114
|
|
EDTA Plasma (n=5)
|
100
|
85-111
|
|
Serum (n=5)
|
99
|
91-108
|
Linearity
To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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