Mouse IL-12 p70 Quantikine ELISA Kit
Mouse IL-12 p70 Quantikine ELISA Kit
Mouse IL-12 p70 Quantikine ELISA Kit Summary
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
貨號：M1270
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-12 p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
36
|
44
|
36
|
|
Mean
|
28
|
68
|
193
|
20
|
56
|
230
|
|
Standard Deviation
|
2.5
|
6.5
|
19.4
|
2.3
|
6.2
|
20
|
|
CV%
|
8.9
|
9.6
|
10.1
|
11.5
|
11.1
|
8.7
|
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
