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          Human CCL17/TARC Quantikine ELISA Kit


          

          
Human CCL17/TARC Quantikine ELISA Kit
          Human CCL17/TARC Quantikine ELISA Kit
          
            Human CCL17/TARC Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	
          
		Human CCL17/TARC Quantikine ELISA Kit Summary
	
          
	
          
		深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
	
          
	
          
		貨號(hào)：DDN00
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					3.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					7 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human TARC
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					87
				
				
					600
				
				
					1107
				
				
					98
				
				
					616
				
				
					1178
				
			
          
			
          
				
					Standard Deviation
				
				
					6.2
				
				
					13.7
				
				
					29.9
				
				
					8.9
				
				
					50.7
				
				
					88.8
				
			
          
			
          
				
					CV%
				
				
					7.1
				
				
					2.3
				
				
					2.7
				
				
					9.1
				
				
					8.2
				
				
					7.5
				
			
          
		          
	
          

          
	
          
		Cell Culture Supernates
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					88
				
				
					504
				
				
					1001
				
				
					89
				
				
					540
				
				
					1033
				
			
          
			
          
				
					Standard Deviation
				
				
					4.1
				
				
					21.7
				
				
					27.2
				
				
					8.3
				
				
					38.9
				
				
					71.7
				
			
          
			
          
				
					CV%
				
				
					4.7
				
				
					4.3
				
				
					2.7
				
				
					9.3
				
				
					7.2
				
				
					6.9
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=4)
				
				
					95
				
				
					88-104
				
			
          
			
          
				
					EDTA Plasma (n=5)
				
				
					102
				
				
					86-111
				
			
          
			
          
				
					Heparin Plasma (n=5)
				
				
					98
				
				
					86-107
				
			
          
			
          
				
					Serum (n=5)
				
				
					105
				
				
					100-114
				
			
          
		          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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