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          Human CD25/IL-2 R alpha Quantikine ELISA Kit


          

          
Human CD25/IL-2 R alpha Quantikine ELISA Kit
          Human CD25/IL-2 R alpha Quantikine ELISA Kit
          
            Human CD25/IL-2 R alpha Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	
          
		Human CD25/IL-2 R alpha Quantikine ELISA Kit Summary
	
          
	
          
		深圳子科生物優(yōu)勢(shì)代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來電咨詢0755-33164177！
	
          
	
          
		貨號(hào)：DR2A00
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					3.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (10 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					10 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human IL-2 sR alpha
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					207
				
				
					613
				
				
					2357
				
				
					188
				
				
					630
				
				
					2615
				
			
          
			
          
				
					Standard Deviation
				
				
					12.7
				
				
					37.5
				
				
					108
				
				
					13.6
				
				
					38.1
				
				
					183
				
			
          
			
          
				
					CV%
				
				
					6.1
				
				
					6.1
				
				
					4.6
				
				
					7.2
				
				
					6
				
				
					7
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of IL-2 sR alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=5)
				
				
					105
				
				
					93-118
				
			
          
			
          
				
					EDTA Plasma (n=5)
				
				
					103
				
				
					91-113
				
			
          
			
          
				
					Heparin Plasma (n=5)
				
				
					104
				
				
					98-113
				
			
          
			
          
				
					Serum (n=5)
				
				
					105
				
				
					99-116
				
			
          
		          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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