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          Human FGF-21 Quantikine ELISA Kit


          

          
Human FGF-21 Quantikine ELISA Kit
          Human FGF-21 Quantikine ELISA Kit
          
            Human FGF-21 Quantikine ELISA Kit
          
            
           詳情說(shuō)明
          
                
          
	
          
		Human FGF-21 Quantikine ELISA Kit Summary
	
          
	
          
		深圳子科生物優(yōu)勢(shì)代理美國(guó)R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來(lái)電咨詢0755-33164177！
	
          
	
          
		貨號(hào):DF2100
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					4.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					8.69 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human FGF-21
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					204
				
				
					672
				
				
					1324
				
				
					245
				
				
					742
				
				
					1411
				
			
          
			
          
				
					Standard Deviation
				
				
					8
				
				
					19.8
				
				
					46.2
				
				
					26.6
				
				
					47.2
				
				
					73.9
				
			
          
			
          
				
					CV%
				
				
					3.9
				
				
					2.9
				
				
					3.5
				
				
					10.9
				
				
					6.4
				
				
					5.2
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=4)
				
				
					98
				
				
					93-108
				
			
          
			
          
				
					EDTA Plasma (n=4)
				
				
					103
				
				
					92-112
				
			
          
			
          
				
					Heparin Plasma (n=4)
				
				
					98
				
				
					83-111
				
			
          
			
          
				
					Serum (n=4)
				
				
					105
				
				
					87-119
				
			
          
		          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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