Mouse/Rat FGF-21 Quantikine ELISA Kit
Mouse/Rat FGF-21 Quantikine ELISA Kit
Mouse/Rat FGF-21 Quantikine ELISA Kit Summary
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貨號(hào)：MF2100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat FGF-21
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
118
|
317
|
1032
|
120
|
302
|
977
|
|
Standard Deviation
|
7.35
|
13.5
|
25.2
|
8.91
|
18
|
65.9
|
|
CV%
|
6.2
|
4.3
|
2.4
|
7.4
|
6
|
6.7
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
84.4
|
226
|
754
|
94.5
|
231
|
745
|
|
Standard Deviation
|
5.24
|
9.6
|
19.7
|
6.61
|
10.8
|
31
|
|
CV%
|
6.2
|
4.2
|
2.6
|
7
|
4.7
|
4.2
|
Recovery
The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Samples (n=6)
|
96
|
88-101
|
|
Mouse EDTA Plasma (n=4)
|
97
|
86-104
|
|
Mouse Heparin Plasma (n=4)
|
93
|
83-101
|
|
Mouse Serum (n=4)
|
95
|
83-103
|
|
Rat Cell Culture Samples (n=2)
|
91
|
87-95
|
|
Rat EDTA Plasma (n=4)
|
101
|
93-119
|
|
Rat Heparin Plasma (n=4)
|
97
|
94-104
|
|
Rat Serum (n=4)
|
100
|
93-108
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
