Human Galectin-3 Quantikine ELISA Kit Summary
Human Galectin-3 Quantikine ELISA Kit Summary
Human Galectin-3 Quantikine ELISA Kit Summary
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貨號：DGAL30
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Galectin-3. This assay cross-reacts 58% with Mouse Galectin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.79
|
2.46
|
5.11
|
0.8
|
2.59
|
5.5
|
|
Standard Deviation
|
0.03
|
0.09
|
0.18
|
0.05
|
0.16
|
0.32
|
|
CV%
|
3.8
|
3.7
|
3.5
|
6.3
|
6.2
|
5.8
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.78
|
2.27
|
5.06
|
0.81
|
2.51
|
5.29
|
|
Standard Deviation
|
0.03
|
0.1
|
0.15
|
0.07
|
0.2
|
0.36
|
|
CV%
|
3.8
|
4.4
|
3
|
8.6
|
8
|
6.8
|
Recovery
The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
96-106
|
|
EDTA Plasma (n=4)
|
105
|
93-113
|
|
Heparin Plasma (n=4)
|
103
|
95-110
|
|
Serum (n=4)
|
104
|
94-115
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
