Human IL-6 R alpha Quantikine ELISA Kit Summary
Human IL-6 R alpha Quantikine ELISA Kit Summary
Human IL-6 R alpha Quantikine ELISA Kit Summary
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貨號(hào)：DR600
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human IL-6 sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
134
|
644
|
1669
|
130
|
645
|
1593
|
|
Standard Deviation
|
11.5
|
16.8
|
38.3
|
8.3
|
26.9
|
75.4
|
|
CV%
|
8.6
|
2.6
|
2.3
|
6.4
|
4.2
|
4.7
|
Recovery
The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
93
|
85-97
|
|
Citrate Plasma (n=4)
|
93
|
86-102
|
|
EDTA Plasma (n=4)
|
93
|
76-107
|
|
Heparin Plasma (n=4)
|
101
|
87-118
|
|
Serum (n=4)
|
93
|
86-110
|
|
Urine (n=4)
|
86
|
64-98
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of natural or recombinant human IL-6 sR were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the as
