Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit Summary
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貨號：MTR00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Free natural and recombinant mouse TRANCE
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse TRANCE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TRANCE in cell culture supernates and serum. It contains NS0-expressed recombinant mouse TRANCE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse TRANCE accurately. Results obtained using natural mouse TRANCE showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TRANCE.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
99
|
261
|
830
|
111
|
285
|
886
|
|
Standard Deviation
|
8
|
6.8
|
18.3
|
8.8
|
17.8
|
57.8
|
|
CV%
|
8.1
|
2.6
|
2.2
|
7.9
|
6.2
|
6.5
|
Recovery
The recovery of mouse TRANCE spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
99
|
87-116
|
|
Serum (n=6)
|
93
|
80-104
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with mouse TRANCE were diluted with Calibrator Diluent and assayed.
