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          Mouse/Rat IL-22 Quantikine ELISA Kit
          Mouse/Rat IL-22 Quantikine ELISA Kit
          Mouse/Rat IL-22 Quantikine ELISA Kit

          Mouse/Rat IL-22 Quantikine ELISA Kit

           詳情說明

          Mouse/Rat IL-22 Quantikine ELISA Kit Summary

          深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒,價(jià)格優(yōu)勢,貨期短,歡迎來電咨詢0755-33164177!

          貨號:M2200

          Assay Type
          Solid Phase Sandwich ELISA
          Format
          96-well strip plate
          Assay Length
          4.5 hours
          Sample Type & Volume Required Per Well
          Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
          Sensitivity
          8.2 pg/mL
          Assay Range
          15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
          Specificity
          Natural and recombinant mouse and rat IL-22
          Cross-reactivity
          < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
          Interference
          Interference observed with 1 or more available related molecules.

          Product Summary

          The Quantikine Mouse/Rat IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-22 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-22 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-22.

          Precision

          Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
          Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

          Cell Culture Supernates

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 35 35 35
          Mean 45.9 129 628 43.9 122 596
          Standard Deviation 1.9 7.5 22.1 3.4 6.7 25.2
          CV% 4.2 5.8 3.5 7.7 5.5 4.2

          Serum, EDTA Plasma, Heparin Plasma

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 34 34 34
          Mean 44.4 117 692 45.4 128 662
          Standard Deviation 2 7 25.7 4.8 7.5 35.5
          CV% 4.4 6 3.7 10.5 5.9 5.4

          Recovery

          The recovery of IL-22 spiked to three levels throughout the range of the assay in various matrices was evaluated.

          Sample Type Average % Recovery Range %
          Mouse Cell Culture Supernates (n=4) 98 93-103
          Mouse EDTA Plasma (n=4) 95 86-106
          Mouse Heparin Plasma (n=4) 94 83-102
          Mouse Serum (n=4) 91 84-99
          Rat Cell Culture Supernates (n=4) 98 91-102
          Rat EDTA Plasma (n=4) 97 91-111
          Rat Heparin Plasma (n=4) 93 88-101
          Rat Serum (n=4) 96 90-107

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