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          Human CCL4/MIP-1 beta Quantikine ELISA Kit
          Human CCL4/MIP-1 beta Quantikine ELISA Kit
          Human CCL4/MIP-1 beta Quantikine ELISA Kit

          Human CCL4/MIP-1 beta Quantikine ELISA Kit

           詳情說明

          Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary

          貨號:DMB00

          深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒,價格優(yōu)勢,貨期短,歡迎來電咨詢0755-33164177!

          Assay Type
          Solid Phase Sandwich ELISA
          Format
          96-well strip plate
          Assay Length
          3.0 hours or 4.5 hours
          Sample Type & Volume Required Per Well
          Cell Culture Supernates (10 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Citrate Plasma (150 uL)
          Sensitivity
          11 pg/mL
          Assay Range
          15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
          Specificity
          Natural and recombinant human MIP-1 beta
          Cross-reactivity
          < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
          Interference
          No significant interference observed with available related molecules.

          Product Summary

          The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Results obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.

          Precision

          Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
          Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

          Cell Culture Supernates

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 40 40 40
          Mean 23.3 98.8 428 31.4 118 476
          Standard Deviation 1.1 3.8 17.3 3.7 9.9 28
          CV% 4.7 3.8 4 11.8 8.4 5.9

          Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 40 40 40
          Mean 51.3 208 932 58.8 220 949
          Standard Deviation 4.6 7.5 30.2 5.7 15.6 70.5
          CV% 9 3.6 3.2 9.7 7.1 7.4

          Recovery

          The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.

          Sample Type Average % Recovery Range %
          Cell Culture Media (n=5) 107 81-117
          Citrate Plasma (n=5) 99 87-116
          EDTA Plasma (n=5) 100 84-112
          Heparin Plasma (n=5) 99 90-112
          Serum (n=5) 99 88-113

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