Human sE-Selectin/CD62E Quantikine ELISA Kit
Human sE-Selectin/CD62E Quantikine ELISA Kit
Human sE-Selectin/CD62E Quantikine ELISA Kit Summary
Catalog Availability Size / Price
DSLE00 1 Kit / Please Inquire
SSLE00 1 Pack (6 Plates) / Please Inquire
PDSLE00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Assay Range
0.1 - 8 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sE-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.6
|
2.9
|
4.7
|
1.5
|
2.9
|
4.6
|
|
Standard Deviation
|
0.11
|
0.17
|
0.24
|
0.11
|
0.25
|
0.35
|
|
CV%
|
6.6
|
5.7
|
5.2
|
7.3
|
8.7
|
7.7
|
Recovery
The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
103
|
98-110
|
|
Citrate Plasma (n=4)
|
96
|
85-107
|
|
Heparin Plasma (n=4)
|
94
|
85-107
|
|
Serum (n=4)
|
96
|
85-106
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
