熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
貨號：DMP100 規(guī)格：96T
Cell Culture Supernates
Serum, Heparin Plasma
The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.095 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human Pro-MMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
0.494
1.65
3.39
0.561
1.73
3.5
Standard Deviation
0.022
0.065
0.177
0.065
0.153
0.27
CV%
4.5
4
5.2
11.7
8.8
7.7
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
0.702
2.13
4.43
0.738
2.34
4.66
Standard Deviation
0.039
0.13
0.241
0.077
0.176
0.312
CV%
5.6
6.1
5.4
10.4
7.5
6.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
103
98-112
Heparin Plasma (n=5)
92
85-102
Serum (n=5)
97
87-112
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
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