Human M-CSF Quantikine ELISA Kit
Human M-CSF Quantikine ELISA Kit
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Human M-CSF Quantikine ELISA Kit Summary
Catalog Size / Price
DMC00B 1 Kit / Please Inquire
SMC00B 1 Pack (6 Plates) / Please Inquire
PDMC00B 1 Pack (50 Plates) / Please Inquire
Specificity
Natural and recombinant human M-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human M-CSF in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human M-CSF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human M-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human M-CSF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
248
|
751
|
1503
|
280
|
827
|
1620
|
|
Standard Deviation
|
19.4
|
16.1
|
49
|
30.2
|
76
|
87.7
|
|
CV%
|
7.8
|
2.1
|
3.3
|
10.7
|
9.2
|
5.4
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
357
|
951
|
1767
|
343
|
1019
|
1956
|
|
Standard Deviation
|
17.4
|
24.9
|
49.4
|
43.4
|
84.1
|
88.8
|
|
CV%
|
4.9
|
2.6
|
2.8
|
12.6
|
8.3
|
4.5
|
Recovery
The recovery of M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
99
|
90-107
|
|
EDTA Plasma (n=4)
|
100
|
93-106
|
|
Heparin Plasma (n=4)
|
99
|
93-106
|
|
Serum (n=4)
|
101
|
96-106
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of M-CSF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
