Human FABP2/I-FABP Quantikine ELISA Kit
Human FABP2/I-FABP Quantikine ELISA Kit
Human FABP2/I-FABP Quantikine ELISA Kit Summary
貨號：DFBP20
規(guī)格：96T
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FABP2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
77.5
|
207
|
425
|
80.5
|
216
|
446
|
|
Standard Deviation
|
3.14
|
7.24
|
12.4
|
8.94
|
17.4
|
26.6
|
|
CV%
|
4.1
|
3.5
|
2.9
|
11.1
|
8.1
|
6
|
Recovery
The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
100
|
96-104
|
|
Cell Lysis Buffer (n=2)
|
83
|
81-85
|
|
EDTA Plasma (n=4)
|
97
|
85-102
|
|
Heparin Plasma (n=4)
|
95
|
96-104
|
|
Serum (n=4)
|
101
|
97-106
|
|
Urine (n=4)
|
90
|
85-95
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
