Human Total MMP-7 Quantikine ELISA Kit
Human Total MMP-7 Quantikine ELISA Kit
Human Total MMP-7 Quantikine ELISA Kit Summary
Catalog Size / Price
DMP700 1 Kit / Please Inquire
SMP700 1 Pack (6 Plates) / Please Inquire
PDMP700 1 Pack (50 Plates) / Please Inquire
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), Heparin Plasma (25 uL), Saliva (25 uL), Urine (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human MMP-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.26
|
4.58
|
6.29
|
1.23
|
4.82
|
6.76
|
|
Standard Deviation
|
0.064
|
0.168
|
0.217
|
0.054
|
0.198
|
0.309
|
|
CV%
|
5.1
|
3.7
|
3.4
|
4.4
|
4.1
|
4.6
|
Recovery
The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
101
|
96-108
|
|
Urine (n=2)
|
99
|
89-109
|
Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
