Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary
貨號：DPTX30B 規(guī)格：96T
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), EDTA Plasma (20 uL), Saliva (20 uL)
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human Pentraxin 3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Pentraxin 3/TSG-14 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human Pentraxin 3 in serum-free cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human Pentraxin 3 and antibodies raised against the recombinant protein. Natural human Pentraxin 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human
Pentraxin 3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Serum-free Cell Culture Supernates, EDTA Plasma, Saliva
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
2.12
|
6.03
|
11.6
|
2.05
|
5.86
|
11.4
|
|
Standard Deviation
|
0.038
|
0.158
|
0.14
|
0.141
|
0.383
|
0.5
|
|
CV%
|
1.8
|
2.6
|
1.2
|
6.9
|
6.5
|
4.4
|
Recovery
The recovery of human Pentraxin 3 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
EDTA Plasma (n=4)
|
99
|
85-108
|
|
Saliva (n=4)
|
89
|
79-99
|
|
Serum-free Cell Culture Media (n=2)
|
109
|
100-120
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
