Human Hepcidin Quantikine ELISA Kit
Human Hepcidin Quantikine ELISA Kit
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Human Hepcidin Quantikine ELISA Kit Summary
貨號：DHP250 規(guī)格：96T
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural human Hepcidin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Hepcidin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Hepcidin in cell culture supernates, serum, plasma, and urine. It contains synthetic Hepcidin and antibodies raised against synthetic Hepcidin. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring human Hepcidin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
175
|
350
|
726
|
164
|
328
|
647
|
|
Standard Deviation
|
7.51
|
10.8
|
23.5
|
18
|
26.1
|
39.8
|
|
CV%
|
4.3
|
3.1
|
3.2
|
11
|
8
|
6.2
|
Recovery
The recovery of human Hepcidin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
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Sample Type
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Average % Recovery
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Range %
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|
Cell Culture Media (n=4)
|
111
|
99-118
|
|
EDTA Plasma (n=4)
|
103
|
95-115
|
|
Heparin Plasma (n=4)
|
104
|
93-117
|
|
Serum (n=4)
|
106
|
96-118
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Hepcidin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
