Human Angiopoietin-like 3 Quantikine ELISA Kit
Human Angiopoietin-like 3 Quantikine ELISA Kit
Human Angiopoietin-like 3 Quantikine ELISA Kit Summary
Catalog # DANL30
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ANGPTL3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human ANGPTL3 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human ANGPTL3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human ANGPTL3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ANGPTL3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ANGPTL3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.05
|
3.36
|
6.8
|
1.02
|
3.18
|
6.74
|
|
Standard Deviation
|
0.04
|
0.09
|
0.18
|
0.07
|
0.2
|
0.6
|
|
CV%
|
4.1
|
2.7
|
2.7
|
6.7
|
7.2
|
8.5
|
Recovery
The recovery of ANGPTL3 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
103
|
99-107
|
|
EDTA Plasma (n=4)
|
102
|
85-120
|
|
Heparin Plasma (n=4)
|
96
|
85-109
|
|
Serum (n=4)
|
96
|
81-115
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of ANGPTL3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
