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          Human SCF Quantikine ELISA Kit


          

          
Human SCF Quantikine ELISA Kit
          Human SCF Quantikine ELISA Kit
          
            Human SCF Quantikine ELISA Kit
          
            
           詳情說明
          
                
          深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！

          
	
          
		
          
			Human SCF Quantikine ELISA Kit Summary
		
          
		
          
			Catalog #DCK00   
		
          
		
          
			
          
				
          
					
          
						Assay Type
					
          
					
          
						Solid Phase Sandwich ELISA
					
          
				
          
				
          
					
          
						Format
					
          
					
          
						96-well strip plate
					
          
				
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						9 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant human SCF. This assay cross-reacts 15.6% with Canine SCF, 16.1% with Feline SCF, 3.5% with Mouse SCF and 1.0% with Rat SCF.
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine Human SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure SCF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human soluble SCF (KL-1 variant) and antibodies raised against this recombinant factor. Results obtained for naturally occurring human SCF samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SCF.
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
		
          
		
          
			Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
		
          
		
          
			Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
				
          
				
          
					
						Mean
					
					
						139
					
					
						655
					
					
						1419
					
					
						142
					
					
						703
					
					
						1574
					
				
          
				
          
					
						Standard Deviation
					
					
						5.5
					
					
						12.9
					
					
						54
					
					
						14.6
					
					
						36
					
					
						94
					
				
          
				
          
					
						CV%
					
					
						4
					
					
						2
					
					
						3.8
					
					
						10.3
					
					
						5.1
					
					
						6
					
				
          
			          
		
          

          
		
          
			Cell Culture Supernates
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
				
          
				
          
					
						Mean
					
					
						153
					
					
						709
					
					
						1427
					
					
						151
					
					
						729
					
					
						1552
					
				
          
				
          
					
						Standard Deviation
					
					
						3.6
					
					
						11.6
					
					
						61
					
					
						12.1
					
					
						45.8
					
					
						125
					
				
          
				
          
					
						CV%
					
					
						2.4
					
					
						1.6
					
					
						4.3
					
					
						8
					
					
						6.3
					
					
						8.1
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of SCF spiked to levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Cell Culture Media (n=2)
					
					
						85
					
					
						75-93
					
				
          
				
          
					
						Citrate Plasma (n=8)
					
					
						94
					
					
						84-112
					
				
          
				
          
					
						EDTA Plasma (n=8)
					
					
						98
					
					
						82-110
					
				
          
				
          
					
						Heparin Plasma (n=8)
					
					
						99
					
					
						88-116
					
				
          
				
          
					
						Serum (n=8)
					
					
						99
					
					
						87-111
					
				
          
			          
		
          
	
          
	
          
		
          
			Linearity
		
          
		
          
			To assess the linearity of the assay, the following biological samples were diluted with the appropriate Calibrator Diluent and then assayed. The cell culture media was spiked with exogenous SCF.
		
          
		
          
			Human SCF/c-kit Ligand ELISA Linearity
		
          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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