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          Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit


          

          
Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit
          Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit
          
            Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit
          
            
           詳情說明
          
                
          深圳子科生物優(yōu)勢(shì)代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來電咨詢0755-33164177！

          
	
          
		
          
			Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit Summary
		
          
		
          
			Catalog：MPC900
		
          
		
          
			
          
				
          
					
          
						Assay Type
					
          
					
          
						Solid Phase Sandwich ELISA
					
          
				
          
				
          
					
          
						Format
					
          
					
          
						96-well strip plate
					
          
				
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (50 uL), Cell Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						21.9 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant mouse PCSK9. This kit detects 60 kDa, 53 kDa, and LDLR-complexed recombinant mouse PCSK9.
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine Mouse PCSK9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse PCSK9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PCSK9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PCSK9.
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
		
          
		
          
			Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
		
          
		
          
			Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						40
					
					
						40
					
					
						40
					
				
          
				
          
					
						Mean
					
					
						181
					
					
						427
					
					
						1427
					
					
						213
					
					
						469
					
					
						1511
					
				
          
				
          
					
						Standard Deviation
					
					
						10.3
					
					
						32.1
					
					
						44.1
					
					
						18.9
					
					
						25.9
					
					
						102
					
				
          
				
          
					
						CV%
					
					
						5.7
					
					
						7.5
					
					
						3.1
					
					
						8.9
					
					
						5.5
					
					
						6.8
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of mouse PCSK9 spiked to three levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Cell Culture Supernates (n=6)
					
					
						102
					
					
						94-115
					
				
          
				
          
					
						Cell Lysates (n=5)
					
					
						103
					
					
						91-117
					
				
          
			          
		
          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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