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          Mouse/Rat IL-33 Quantikine ELISA Kit


          

          
Mouse/Rat IL-33 Quantikine ELISA Kit
          Mouse/Rat IL-33 Quantikine ELISA Kit
          
            Mouse/Rat IL-33 Quantikine ELISA Kit
          
            
           詳情說(shuō)明
          
                
          
	深圳子科生物優(yōu)勢(shì)代理美國(guó)R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來(lái)電咨詢(xún)0755-33164177！

          

          
	
          
		
          
			Mouse/Rat IL-33 Quantikine ELISA Kit Summary
		
          
		
          
			貨號(hào):M3300
		
          
		
          
			
          
				
          
					
          
						Assay Type
					
          
					
          
						Solid Phase Sandwich ELISA
					
          
				
          
				
          
					
          
						Format
					
          
					
          
						96-well strip plate
					
          
				
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						14.3 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant mouse and rat IL-33
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natural IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
		
          
		
          
			Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
		
          
		
          
			Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						41
					
					
						41
					
					
						41
					
				
          
				
          
					
						Mean
					
					
						58.2
					
					
						298
					
					
						630
					
					
						57.6
					
					
						282
					
					
						599
					
				
          
				
          
					
						Standard Deviation
					
					
						5.6
					
					
						17.4
					
					
						33.5
					
					
						5.89
					
					
						16.3
					
					
						30
					
				
          
				
          
					
						CV%
					
					
						9.6
					
					
						5.8
					
					
						5.3
					
					
						10.2
					
					
						5.8
					
					
						5
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of IL-33 spiked to three levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Mouse Cell Culture Supernates (n=4)
					
					
						104
					
					
						91-116
					
				
          
				
          
					
						Mouse EDTA Plasma (n=4)
					
					
						102
					
					
						96-113
					
				
          
				
          
					
						Mouse Heparin Plasma (n=4)
					
					
						99
					
					
						83-111
					
				
          
				
          
					
						Mouse Serum (n=4)
					
					
						90
					
					
						81-103
					
				
          
				
          
					
						Rat Cell Culture Supernates (n=4)
					
					
						104
					
					
						94-116
					
				
          
				
          
					
						Rat EDTA Plasma (n=4)
					
					
						93
					
					
						83-100
					
				
          
				
          
					
						Rat Heparin Plasma (n=4)
					
					
						93
					
					
						87-99
					
				
          
				
          
					
						Rat Serum (n=4)
					
					
						93
					
					
						86-101
					
				
          
			          
		
          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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