Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Human Coagulation Factor III/Tissue Factor Quantikine ELISA Summary
貨號：DCF300
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), Urine (50 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human Coagulation Factor III
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
33.4
|
65.4
|
148
|
71
|
144
|
301
|
|
Standard Deviation
|
0.78
|
1.81
|
5
|
4.5
|
7.8
|
17.1
|
|
CV%
|
2.3
|
2.8
|
3.4
|
6.3
|
5.4
|
5.7
|
Recovery
The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
104
|
95-110
|
|
Citrate Plasma (n=4)
|
92
|
86-102
|
|
EDTA Plasma (n=4)
|
91
|
86-97
|
|
Heparin Plasma (n=4)
|
95
|
88-109
|
|
Urine (n=4)
|
102
|
96-112
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
