熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
貨號：MSAA00 規(guī)格：96T
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of mouse SAA spiked to levels throughout the range of the assay was evaluated.
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.022 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse SAA.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Mouse Serum Amyloid A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SAA in cell culture supernates, tissue culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse SAA1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SAA. Results obtained using natural mouse SAA showed dose response curves that were parallel to the standard curves obtained using the Quantikine® mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SAA.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
0.413
1.35
2.35
0.459
1.35
2.51
Standard Deviation
0.02
0.073
0.11
0.036
0.082
0.178
CV%
4.8
5.4
4.7
7.8
6.1
7.1
Recovery
Sample Type
Average % Recovery
Range %
Culture Media (n=4)
93
84-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SAA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
0755-28715175/33164177
粵公網(wǎng)安備 44030902000304號
