Human Growth Hormone Quantikine ELISA Kit
Human Growth Hormone Quantikine ELISA Kit
Human Growth Hormone Quantikine ELISA Kit Summary
Catalog # Availability Size / Price
DGH00 1 Kit / Please Inquire
SGH00 1 Pack (6 Plates) / Please Inquire
PDGH00 1 Pack (50 Plates) / Please Inquire
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Growth Hormone
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
201
|
595
|
1091
|
209
|
623
|
1152
|
|
Standard Deviation
|
9.4
|
18.8
|
26
|
18.2
|
45.6
|
63.6
|
|
CV%
|
4.7
|
3.2
|
2.4
|
8.7
|
7.3
|
5.5
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
197
|
586
|
1092
|
215
|
639
|
1189
|
|
Standard Deviation
|
8
|
13.8
|
29.8
|
20.2
|
49.5
|
82.2
|
|
CV%
|
4.1
|
2.4
|
2.7
|
9.4
|
7.7
|
6.9
|
