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          Human Relaxin-2 Quantikine ELISA Kit


          

          
Human Relaxin-2 Quantikine ELISA Kit
          Human Relaxin-2 Quantikine ELISA Kit
          
            Human Relaxin-2 Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！

          

          
	貨號：DRL200   規(guī)格：96T

          

          
	
          
		
          
			Human Relaxin-2 Quantikine ELISA Kit Summary
		
          
		
          
			
          
				
          
					
          
						Assay Type
					
          
					
          
						Solid Phase Sandwich ELISA
					
          
				
          
				
          
					
          
						Format
					
          
					
          
						96-well strip plate
					
          
				
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						4.57 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant human Relaxin-2
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine Human Relaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Relaxin-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Relaxin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Relaxin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Relaxin-2.
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
		
          
		
          
			Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
		
          
		
          
			Serum, EDTA Plasma, Heparin Plasma
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						42
					
					
						42
					
					
						42
					
				
          
				
          
					
						Mean
					
					
						34.4
					
					
						99.6
					
					
						206
					
					
						40.8
					
					
						112
					
					
						220
					
				
          
				
          
					
						Standard Deviation
					
					
						1.6
					
					
						2.47
					
					
						4.75
					
					
						4.16
					
					
						7.02
					
					
						12.1
					
				
          
				
          
					
						CV%
					
					
						4.7
					
					
						2.5
					
					
						2.3
					
					
						10.2
					
					
						6.3
					
					
						5.5
					
				
          
			          
		
          

          
		
          
			Cell Culture Supernates
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						41
					
					
						41
					
					
						41
					
				
          
				
          
					
						Mean
					
					
						35.1
					
					
						96
					
					
						194
					
					
						34.9
					
					
						98.9
					
					
						197
					
				
          
				
          
					
						Standard Deviation
					
					
						1.27
					
					
						2.44
					
					
						4.01
					
					
						3.41
					
					
						5.9
					
					
						11.7
					
				
          
				
          
					
						CV%
					
					
						3.6
					
					
						2.5
					
					
						2.1
					
					
						9.8
					
					
						6
					
					
						5.9
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of Relaxin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Cell Culture Media (n=4)
					
					
						95
					
					
						89-102
					
				
          
				
          
					
						EDTA Plasma (n=4)
					
					
						98
					
					
						93-106
					
				
          
				
          
					
						Heparin Plasma (n=4)
					
					
						97
					
					
						91-104
					
				
          
				
          
					
						Serum (n=4)
					
					
						101
					
					
						92-107
					
				
          
			          
		
          
	
          

          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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