Mouse/Rat PDGF-BB Quantikine ELISA Kit
Mouse/Rat PDGF-BB Quantikine ELISA Kit
Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary
貨號：MBB00 規(guī)格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat PDGF-BB. This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
22
|
24
|
24
|
|
Mean
|
49.7
|
252
|
823
|
52.1
|
246
|
811
|
|
Standard Deviation
|
4.5
|
18.7
|
36.7
|
5.3
|
21.1
|
40.4
|
|
CV%
|
9.1
|
7.4
|
4.5
|
10.2
|
8.6
|
5
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
22
|
22
|
23
|
|
Mean
|
61.1
|
298
|
1019
|
69.5
|
305
|
1048
|
|
Standard Deviation
|
5.6
|
24.6
|
40.3
|
6.6
|
26.3
|
98.5
|
|
CV%
|
9.2
|
8.3
|
4
|
9.5
|
8.6
|
9.4
|
Recovery
The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=8)
|
100
|
89-118
|
|
Mouse Heparin Plasma (n=5)
|
96
|
85-113
|
|
Rat Cell Culture Supernates (n=8)
|
102
|
94-114
|
|
Rat EDTA Plasma (n=5)
|
102
|
86-111
|
|
Rat Heparin Plasma (n=5)
|
102
|
85-111
|
|
Rat Serum (n=5)
|
100
|
89-113
|
Linearity
To assess the linearity of the assay, samples spiked with or containing PDGF-BB in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
