Human SP-D Quantikine ELISA Kit
Human SP-D Quantikine ELISA Kit
Human SP-D Quantikine ELISA Kit Summary
貨號：DSFPD0
規(guī)格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human SP-D
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human SP-D Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human SP-D in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SP-D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SP-D showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring SP-D.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
6.07
|
11.7
|
23.4
|
5.63
|
11.2
|
22.8
|
|
Standard Deviation
|
0.495
|
0.588
|
1.46
|
0.495
|
0.999
|
1.78
|
|
CV%
|
8.2
|
5
|
6.2
|
8.8
|
8.9
|
7.8
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
5.99
|
11.3
|
24.3
|
6.63
|
13.1
|
26.9
|
|
Standard Deviation
|
0.371
|
0.921
|
1.63
|
0.617
|
1.14
|
2.5
|
|
CV%
|
6.2
|
8.2
|
6.7
|
9.3
|
8.7
|
9.3
|
Recovery
The recovery of SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
95-109
|
|
EDTA Plasma (n=4)
|
110
|
99-120
|
|
Heparin Plasma (n=4)
|
104
|
85-118
|
|
Serum (n=4)
|
106
|
89-118
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SP-D were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
