Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
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Human t-Plasminogen Activator/tPA Quantikine ELISA Kit Summary
貨號：DTPA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Citrate Plasma (13 uL), Saliva (50 uL), Urine (25 uL), Human Milk (13 uL)
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human tPA. This assay also recognizes tPA complexed with PAI-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human t-Plasminogen Activator/tPA Immunoassay is a 4.5 hour solid phase ELISA designed to measure tPA levels in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human tPA and antibodies raised against the recombinant protein. Results obtained for naturally occurring human tPA showed linear curves that were parallel to the standard curves obtained using the Quantikine Human tPA Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human tPA.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Saliva, Urine, Human Milk
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
498
|
1475
|
2878
|
490
|
1460
|
2898
|
|
Standard Deviation
|
36
|
48.4
|
88.8
|
32.1
|
62.3
|
115
|
|
CV%
|
7.2
|
3.3
|
3.1
|
6.6
|
4.3
|
4
|
Recovery
The recovery of human tPA spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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|
Cell Culture Media (n=4)
|
93
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86-97
|
|
Citrate Plasma (n=4)
|
86
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80-98
|
|
EDTA Plasma (n=4)
|
87
|
80-97
|
|
Heparin Plasma (n=4)
|
86
|
79-100
|
|
Serum (n=4)
|
95
|
87-109
|
|
Urine (n=4)
|
97
|
88-110
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human tPA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
