Human Osteocalcin Quantikine ELISA Kit
Human Osteocalcin Quantikine ELISA Kit
Human Osteocalcin Quantikine ELISA Kit Summary
貨號：DSTCN0
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (50 uL)
Assay Range
2.0 - 64 ng/mL (Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant human Osteocalcin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Osteocalcin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteocalcin in cell culture supernates and EDTA plasma. It contains synthetic human Osteocalcin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteocalcin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteocalcin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
4
|
4
|
4
|
4
|
4
|
4
|
|
Mean
|
8.15
|
17.2
|
33.3
|
9.06
|
19.6
|
40
|
|
Standard Deviation
|
0.268
|
0.395
|
0.544
|
0.306
|
0.379
|
0.653
|
|
CV%
|
3.3
|
2.3
|
1.6
|
3.4
|
1.9
|
1.6
|
EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
4
|
4
|
4
|
4
|
4
|
4
|
|
Mean
|
9.06
|
19.6
|
40
|
7.91
|
16.1
|
33.6
|
|
Standard Deviation
|
0.306
|
0.379
|
0.653
|
0.766
|
0.991
|
1.66
|
|
CV%
|
3.4
|
1.9
|
1.6
|
9.7
|
6.2
|
4.9
|
Recovery
The recovery of human Osteocalcin spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
105
|
98-120
|
|
EDTA Plasma (n=4)
|
102
|
80-116
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Osteocalcin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
