Human MFG-E8 Quantikine ELISA Kit
Human MFG-E8 Quantikine ELISA Kit
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Human MFG-E8 Quantikine ELISA Kit Summary
Catalog # DFGE80
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (50 uL), Human Milk (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human MFG-E8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) <div>Three samples of a known concentration were tested in twenty separate
assays to assess inter-assay precision. Assays
were performed by at least three technicians using two lots of kit components. </div>
Cell Culture Supernates, Urine, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
680
|
1181
|
2103
|
692
|
1262
|
2206
|
|
Standard Deviation
|
30.5
|
38.9
|
84.7
|
57.6
|
88
|
125
|
|
CV%
|
4.5
|
3.3
|
4
|
8.3
|
7
|
5.7
|
Serum, EDTA Plasma, Heparin Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
645
|
1143
|
2168
|
749
|
1326
|
2339
|
|
Standard Deviation
|
39.5
|
46.6
|
81.1
|
57.4
|
78
|
117
|
|
CV%
|
6.1
|
4.1
|
3.7
|
7.7
|
5.9
|
5
|
Recovery
The recovery of human MFG-E8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
105
|
100-110
|
|
EDTA Plasma (n=4)
|
96
|
89-102
|
|
Heparin Plasma (n=4)
|
96
|
89-102
|
|
Serum (n=4)
|
101
|
92-110
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of MFG-E8 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
