Human SHBG Quantikine ELISA Kit
Human SHBG Quantikine ELISA Kit
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Human SHBG Quantikine ELISA Kit Summary
貨號(hào)：DSHBG0B 規(guī)格：96T
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.1 - 4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human SHBG.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SHBG.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
|
2
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3
|
|
n
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20
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20
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20
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20
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20
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20
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|
Mean
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0.565
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1.36
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2.72
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0.562
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1.35
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2.71
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Standard Deviation
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0.032
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0.04
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0.097
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0.042
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0.079
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0.131
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CV%
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5.7
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3
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3.6
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7.6
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5.8
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4.8
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Recovery
The recovery of human SHBG spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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92
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87-96
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SHBG were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
