Human FABP4 Quantikine ELISA Kit
Human FABP4 Quantikine ELISA Kit
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Human FABP4 Quantikine ELISA Kit Summary
貨號：DFBP40
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human FABP4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human FABP4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FABP4 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human FABP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FABP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FABP4.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
349
|
1155
|
2595
|
407
|
1236
|
2619
|
|
Standard Deviation
|
20.2
|
39.7
|
117
|
51.6
|
156
|
291
|
|
CV%
|
5.8
|
3.4
|
4.5
|
12.7
|
12.6
|
11.1
|
Recovery
The recovery of human FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
108
|
100-118
|
|
Cell Lysis Buffer 1 (n=2)
|
108
|
92-117
|
|
EDTA Plasma (n=4)
|
98
|
88-113
|
|
Heparin Plasma (n=4)
|
95
|
83-103
|
|
Serum (n=4)
|
99
|
82-113
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
