Porcine IFN-gamma Quantikine ELISA Kit
Porcine IFN-gamma Quantikine ELISA Kit
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Porcine IFN-gamma Quantikine ELISA Kit Summary
貨號(hào)：PIF00 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant porcine IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Porcine IFN-gamma Immunoassay is a 4 hour solid-phase ELISA designed to measure porcine IFN-gamma in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring porcine IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IFN-gamma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
29
|
34
|
34
|
|
Mean
|
124
|
272
|
913
|
120
|
282
|
904
|
|
Standard Deviation
|
5.8
|
7.4
|
26.5
|
14
|
15.7
|
84.7
|
|
CV%
|
4.7
|
2.7
|
2.9
|
11.7
|
5.6
|
9.4
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
36
|
32
|
36
|
|
Mean
|
226
|
449
|
1518
|
227
|
515
|
1522
|
|
Standard Deviation
|
10.1
|
12
|
40.4
|
17.1
|
51.3
|
97.7
|
|
CV%
|
4.5
|
2.7
|
2.7
|
7.5
|
10
|
6.4
|
Recovery
The recovery of porcine IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
97
|
84-116
|
|
EDTA Plasma (n=5)
|
98
|
85-111
|
|
Heparin Plasma (n=5)
|
106
|
86-118
|
|
Serum (n=5)
|
98
|
81-119
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of porcine IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
