Mouse ST2/IL-33 R Quantikine ELISA Kit
Mouse ST2/IL-33 R Quantikine ELISA Kit
Mouse ST2/IL-33 R Quantikine ELISA Kit Summary
貨號(hào)：MST200
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse ST2
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse ST2/IL-33 R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.465
|
1.34
|
3.81
|
0.512
|
1.43
|
3.94
|
|
Standard Deviation
|
0.019
|
0.042
|
0.106
|
0.047
|
0.082
|
0.138
|
|
CV%
|
4.1
|
3.1
|
2.8
|
9.2
|
5.7
|
3.5
|
Recovery
The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
98
|
81-111
|
|
EDTA Plasma (n=4)
|
104
|
92-118
|
|
Heparin Plasma (n=4)
|
94
|
86-103
|
|
Serum (n=4)
|
103
|
89-113
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
