Human Proinsulin Quantikine ELISA Kit
Human Proinsulin Quantikine ELISA Kit
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Human Proinsulin Quantikine ELISA Kit Summary
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Catalog #
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Availability
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Size / Price
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DPINS0
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Contact your distributor for availability.
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1 Kit / Please Inquire
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
3.3 - 210 pM (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Proinsulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Proinsulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Proinsulin in serum and plasma. It contains E. coli-expressed recombinant human Proinsulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Proinsulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Proinsulin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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21.1
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62.7
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119
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18
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55.1
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109
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Standard Deviation
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0.44
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0.745
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1.4
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1.9
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3.97
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6.91
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CV%
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2.1
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1.2
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1.2
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10.6
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7.2
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6.3
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Recovery
The recovery of human Proinsulin spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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EDTA Plasma (n=4)
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92
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86-108
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Heparin Plasma (n=4)
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94
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90-100
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Serum (n=4)
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95
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80-105
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Proinsulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
