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          Human Axl Quantikine ELISA Kit


          

          
Human Axl Quantikine ELISA Kit
          Human Axl Quantikine ELISA Kit
          
            Human Axl Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	深圳子科生物優(yōu)勢(shì)代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來電咨詢0755-33164177！

          

          
	
          
		Human Axl Quantikine ELISA Kit Summary
	
          
	
          
		
			
          
				
					Catalog #
				
				
					Availability
				
				
					Size / Price
				
			
          
		          
		
			
          
				
					DAXL00
				
				
					Contact your distributor for availability.
				
				
					1 Kit / Please Inquire
				
			
          
		          
	
          
	
          
		
          
			
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						4.6 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant human Axl
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine® Human Axl Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Axl in cell culture supernates, cell lysates, cerebrospinal fluid, serum, plasma, saliva, and urine. It contains 
          
NS0-expressed recombinant human Axl and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Axl showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Axl.          
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
		
          
		
          
			Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>
		
          
		
          
			Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
				
          
				
          
					
						Mean
					
					
						249
					
					
						565
					
					
						1115
					
					
						258
					
					
						582
					
					
						1131
					
				
          
				
          
					
						Standard Deviation
					
					
						4.21
					
					
						10
					
					
						18.6
					
					
						11.3
					
					
						19.4
					
					
						42.4
					
				
          
				
          
					
						CV%
					
					
						1.7
					
					
						1.8
					
					
						1.7
					
					
						4.4
					
					
						3.3
					
					
						3.7
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of human Axl spiked to levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Cell Culture Media (n=4)
					
					
						89
					
					
						81-98
					
				
          
				
          
					
						Cell Lysis Buffer (n=1)
					
					
						90
					
					
						88-93
					
				
          
				
          
					
						Saliva (n=4)
					
					
						94
					
					
						82-101
					
				
          
			          
		
          
	
          
	
          
		
          
			Linearity
		
          
		
          
			To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Axl were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
		
          
		
          
			Human Axl ELISA Linearity
		
          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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