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          Human EGF Quantikine ELISA Kit Summary


          

          
Human EGF Quantikine ELISA Kit Summary
          Human EGF Quantikine ELISA Kit Summary
          
            Human EGF Quantikine ELISA Kit Summary
          
            
           詳情說明
          
                
          
	
          
		Human EGF Quantikine ELISA Kit Summary
	
          
	
          
		貨號：DEG00
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					3.5 hours or 4.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					0.7 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human EGF
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates, Urine
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
			
          
			
          
				
					Mean
				
				
					15
				
				
					78.9
				
				
					134
				
				
					15
				
				
					80
				
				
					136
				
			
          
			
          
				
					Standard Deviation
				
				
					0.4
				
				
					2.5
				
				
					4
				
				
					0.9
				
				
					4.5
				
				
					6.8
				
			
          
			
          
				
					CV%
				
				
					2.7
				
				
					3.2
				
				
					3
				
				
					6
				
				
					5.6
				
				
					5
				
			
          
		          
	
          

          
	
          
		Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
			
          
			
          
				
					Mean
				
				
					13.5
				
				
					80
				
				
					133
				
				
					14.7
				
				
					77
				
				
					134
				
			
          
			
          
				
					Standard Deviation
				
				
					0.6
				
				
					2
				
				
					2.7
				
				
					0.9
				
				
					3.3
				
				
					6.1
				
			
          
			
          
				
					CV%
				
				
					4.4
				
				
					2.5
				
				
					2
				
				
					6.1
				
				
					4.3
				
				
					4.6
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=5)
				
				
					98
				
				
					88-111
				
			
          
			
          
				
					Citrate Plasma (n=10)
				
				
					104
				
				
					89-115
				
			
          
			
          
				
					EDTA Plasma (n=10)
				
				
					100
				
				
					80-111
				
			
          
			
          
				
					Heparin Plasma (n=10)
				
				
					96
				
				
					79-108
				
			
          
			
          
				
					Serum (n=10)
				
				
					97
				
				
					80-106
				
			
          
			
          
				
					Urine (n=1)
				
				
					102
				
				
					97-108
				
			
          
		          
	
          
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