Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit Summary
貨號(hào)：DHYAL0
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.53
|
4.81
|
14.3
|
1.68
|
4.68
|
14.98
|
|
Standard Deviation
|
0.11
|
0.26
|
0.52
|
0.08
|
0.23
|
0.72
|
|
CV%
|
7.2
|
5.4
|
3.6
|
4.8
|
4.9
|
4.8
|
Recovery
The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=2)
|
104
|
96-116
|
|
EDTA Plasma (n=10)
|
97
|
83-106
|
|
Heparin Plasma (n=10)
|
99
|
80-124
|
|
Serum (n=10)
|
98
|
81-119
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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