Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
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貨號：DLA50
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
28.6
|
125
|
185
|
27.9
|
123
|
187
|
|
Standard Deviation
|
0.4
|
1.4
|
4
|
1.2
|
4.7
|
6.3
|
|
CV%
|
1.4
|
1.1
|
2.2
|
4.3
|
3.8
|
3.4
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
26.9
|
122
|
191
|
20.5
|
60
|
121
|
|
Standard Deviation
|
0.4
|
4.6
|
6.6
|
1.7
|
3.1
|
5.2
|
|
CV%
|
1.5
|
3.8
|
3.5
|
8.3
|
5.2
|
4.3
|
Recovery
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
96
|
91-104
|
|
Citrate Plasma (n=6)
|
97
|
71-119
|
|
EDTA Plasma (n=6)
|
104
|
84-121
|
|
Heparin Plasma (n=6)
|
104
|
72-126
|
|
Serum (n=6)
|
107
|
74-127
|
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
