Human Osteopontin (OPN) Quantikine ELISA Kit Summary
Human Osteopontin (OPN) Quantikine ELISA Kit Summary
Human Osteopontin (OPN) Quantikine ELISA Kit Summary
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貨號(hào)：DOST00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human Osteopontin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
2.3
|
4.9
|
9.3
|
2.36
|
4.81
|
9.17
|
|
Standard Deviation
|
0.092
|
0.126
|
0.267
|
0.155
|
0.274
|
0.499
|
|
CV%
|
4
|
2.6
|
2.9
|
6.6
|
5.7
|
5.4
|
Recovery
The recovery of Osteopontin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
104
|
86-113
|
|
EDTA Plasma (n=4)
|
102
|
92-114
|
|
Heparin Plasma (n=4)
|
101
|
95-112
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Osteopontin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
