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          Total MMP-2 Quantikine ELISA Kit Summary


          

          
Total MMP-2 Quantikine ELISA Kit Summary
          Total MMP-2 Quantikine ELISA Kit Summary
          
            Total MMP-2 Quantikine ELISA Kit Summary
          
            
           詳情說明
          
                
          
	
          
		Total MMP-2 Quantikine ELISA Kit Summary
	
          
	
          
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		貨號：MMP200
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					4.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					0.082 ng/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					0.5 - 32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
	
          
	
          
		Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
				
					20
				
			
          
			
          
				
					Mean
				
				
					4.3
				
				
					11.2
				
				
					22.8
				
				
					4.26
				
				
					11.1
				
				
					21.5
				
			
          
			
          
				
					Standard Deviation
				
				
					0.302
				
				
					0.42
				
				
					0.828
				
				
					0.275
				
				
					0.738
				
				
					1.51
				
			
          
			
          
				
					CV%
				
				
					7
				
				
					3.8
				
				
					3.6
				
				
					6.5
				
				
					6.6
				
				
					7
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=4)
				
				
					99
				
				
					90-105
				
			
          
		          
	
          

          

          
	
          
		Linearity
	
          
	
          
		To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
	
          
	
          
		Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Linearity
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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