Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary
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貨號(hào)：MCX120
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse SDF-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.09
|
2.6
|
4.1
|
1.25
|
3.15
|
4.91
|
|
Standard Deviation
|
0.04
|
0.12
|
0.21
|
0.09
|
0.24
|
0.37
|
|
CV%
|
3.7
|
4.6
|
5.1
|
7.2
|
7.6
|
7.5
|
Recovery
The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
101
|
98-104
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
