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          Rat VEGF Quantikine ELISA Kit


          

          
Rat VEGF Quantikine ELISA Kit
          Rat VEGF Quantikine ELISA Kit
          
            Rat VEGF Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	
          
		Rat VEGF Quantikine ELISA Kit Summary
	
          
	
          
		深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
	
          
	
          
		貨號：RRV00
	
          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					3.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					25 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant rat VEGF. This assay cross-reacts 59% with Mouse VEGF120 (E.Coli-expressed), 73% with Mouse VEGF164 (Sf21-expressed), 14% with Canine VEGF164 (E.Coli-expressed), 26% with Human VEGF121 (Sf21-expressed), 31% with Human VEGF121 (E.Coli-expressed), 24% with Mouse VEGF162 (NS0-expressed), 0.2% with Human VEGF206 (E.Coli-expressed) and  5.2% with Human VEGF/PIGF.
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					Interference observed with 1 or more available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					71
				
				
					65
				
				
					71
				
			
          
			
          
				
					Mean
				
				
					103
				
				
					191
				
				
					891
				
				
					107
				
				
					233
				
				
					900
				
			
          
			
          
				
					Standard Deviation
				
				
					3.8
				
				
					10.7
				
				
					19.7
				
				
					8.5
				
				
					23.3
				
				
					41.1
				
			
          
			
          
				
					CV%
				
				
					3.7
				
				
					5.6
				
				
					2.2
				
				
					7.9
				
				
					10
				
				
					4.6
				
			
          
		          
	
          

          
	
          
		Cell Culture Supernates
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					65
				
				
					60
				
				
					65
				
			
          
			
          
				
					Mean
				
				
					79.4
				
				
					174
				
				
					797
				
				
					87.6
				
				
					204
				
				
					828
				
			
          
			
          
				
					Standard Deviation
				
				
					5.7
				
				
					7
				
				
					14.1
				
				
					8.2
				
				
					21.2
				
				
					42.5
				
			
          
			
          
				
					CV%
				
				
					7.2
				
				
					4
				
				
					1.8
				
				
					9.4
				
				
					10.4
				
				
					5.1
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of rat VEGF spiked to three levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Supernates (n=6)
				
				
					103
				
				
					94-108
				
			
          
			
          
				
					EDTA Plasma (n=6)
				
				
					102
				
				
					95-108
				
			
          
			
          
				
					Heparin Plasma (n=6)
				
				
					92
				
				
					80-100
				
			
          
			
          
				
					Serum (n=6)
				
				
					99
				
				
					90-116
				
			
          
		          
	
          

          

          
	
          
		Linearity
	
          
	
          
		To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat VEGF in each matrix were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
	
          
	
          
		Rat VEGF ELISA Linearity
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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