BMP-2 Quantikine ELISA Kit
BMP-2 Quantikine ELISA Kit
BMP-2 Quantikine ELISA Kit Summary
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貨號：DBP200
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Bone Extracts)
Specificity
Natural and mammalian-expressed recombinant human BMP-2. This assay does not recognize E. coli-expressed BMP-2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Bone Extracts
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
323
|
1010
|
2313
|
344
|
1043
|
2213
|
|
Standard Deviation
|
8.3
|
28.3
|
56
|
25.2
|
55.4
|
140.2
|
|
CV%
|
2.6
|
2.8
|
2.4
|
7.3
|
5.3
|
6.3
|
Recovery
The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
2 M Guanidine-HCI (n=1)
|
98
|
94-101
|
|
2 M Urea (n=1)
|
101
|
92-106
|
|
Cell Culture Media (n=4)
|
102
|
94-110
|
Linearity
The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.
